Composite
Part:BBa_K3790235:Design
Designed by: Chongwen Cao Group: iGEM21_Fudan (2021-10-17)
T7 gene product 5.7 controlled by pBAD+dps promoter fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The following primers were used for constructing this part.
gp5.7_coding1 5-GTCCAACTATGTACGGAACAATGCGAGCCTCGTAGCGGAGGCCGCTTCCCGTGGTCAC gp5.7_coding2 5-CTGCAAGCAATCAAAAGTTGCCCTAAGACTTTCCAGTCCAACTATGTACGGAACAATGC gp5.7_coding3 5-ATGTCTGACTACCTGAAAGTGCTGCAAGCAATCAAAAGTTGCC gp5.7_template1 5-CCCAAGCGCCACCGTTACGTCCgCTAGTAGTCAGGCACGAGATGTGACCACGGGAAGCG gp5.7_template2 5-CATTCGTTTCAGAAAGCGAGTACCGGAAGCAGTGATTTCCCAAGCGCCACCG gp5.7_template3 5-TTAGACACATCCTCCCATTCGTTTCAGAAAGCGAGTACC f_pBAD_dps1 5-CAGCTGTCCCTCCTGTTCAG f_pBAD_dps2 5-CACCGGCTATGTGTTCCGCTATTCggatccgctaatcttatggataaaaatgc f_pBAD_GFP 5-ggtgagccagtgtgaATGCATGCGTAAAGGAGAAGAACTTTTCAC f_pBADtran_tem 5-CAGACATtttttttacctccttaactctagtagctag r_pBAD_dps1 5-GCGGAACACATAGCCGGTGCTATACTTAATCTCcatacccgtttttttgggctagctac r_pBAD_dps2 5-CTGAACAGGAGGGACAGCTG r_pBAD_GFP 5-ggaggtaaaaaaaATGTCTGTTATTTGTATAGTTCATCCATGCCATGTGT r_pBADtran_tem 5-GCATtcacactggctcaccttc