Composite

Part:BBa_K3790235:Design

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-17)


T7 gene product 5.7 controlled by pBAD+dps promoter fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The following primers were used for constructing this part.

gp5.7_coding1  	5-GTCCAACTATGTACGGAACAATGCGAGCCTCGTAGCGGAGGCCGCTTCCCGTGGTCAC 	
gp5.7_coding2  	5-CTGCAAGCAATCAAAAGTTGCCCTAAGACTTTCCAGTCCAACTATGTACGGAACAATGC	
gp5.7_coding3  	5-ATGTCTGACTACCTGAAAGTGCTGCAAGCAATCAAAAGTTGCC                	
gp5.7_template1	5-CCCAAGCGCCACCGTTACGTCCgCTAGTAGTCAGGCACGAGATGTGACCACGGGAAGCG	
gp5.7_template2	5-CATTCGTTTCAGAAAGCGAGTACCGGAAGCAGTGATTTCCCAAGCGCCACCG       	
gp5.7_template3	5-TTAGACACATCCTCCCATTCGTTTCAGAAAGCGAGTACC
f_pBAD_dps1	5-CAGCTGTCCCTCCTGTTCAG
f_pBAD_dps2	5-CACCGGCTATGTGTTCCGCTATTCggatccgctaatcttatggataaaaatgc
f_pBAD_GFP	5-ggtgagccagtgtgaATGCATGCGTAAAGGAGAAGAACTTTTCAC
f_pBADtran_tem	5-CAGACATtttttttacctccttaactctagtagctag
r_pBAD_dps1	5-GCGGAACACATAGCCGGTGCTATACTTAATCTCcatacccgtttttttgggctagctac
r_pBAD_dps2	5-CTGAACAGGAGGGACAGCTG
r_pBAD_GFP	5-ggaggtaaaaaaaATGTCTGTTATTTGTATAGTTCATCCATGCCATGTGT
r_pBADtran_tem	5-GCATtcacactggctcaccttc

Source

References