Composite

Part:BBa_K3790226:Design

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-17)


GFP controlled by σS promoter csiD_improved


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707


Design Notes

The following primers were used for constructing this part. 

F_csiD2_1035	TTTTCAGAAATCTAGCTATTTTTAGATTAACTAGAGAAAGAGGAGAAATACTAGATGC	
F_csiD2_1907	ATATGGCCTTAAGGCCTATATGATGACACACATTAACAAATTTCGTG           	
R_csiD2_1035	TATAGGCCTTAAGGCCATATCCTGCCAAGCTCTCAGG                     	
R_csiD2_1907	AATAGCTAGATTTCTGAAAAATGCGCACACTCCCACTCCACCTGAAC

Source

References