Part:BBa_K3790226
GFP controlled by σS promoter csiD_improved
RNA polymerase in E. coli. relies on combined with σ factors to recognize promoter sequences. Although σ70 factor is responsible for the transcription of most of the genes in the genome. E. coli has other six kinds of alternative σ factors that are triggered in stressful conditions. By switching σ factors, bacteria can dramatically change the whole transcription pattern in order to express specific proteins to help themself survive in a bad day.
Among the six alternative σ factors, σS factor recognizes DNA sequences that are very similar to the typical promoter sequences recognized by σ70. Therefore, it is thought that σS promoters rely on upstream elements[1], which are protein-binding DNA sequences distributed upstream, to show their dependence on σS factors.
We noticed that there’s no standard nor well-characterized σS promoter in the registry. Therefore, we tested the core regions of several σS promoters and examined where they can be recognized by σS factors independently.This is one of the devices used to test σS promoters.
Usage
It can be used to generate GFP during plateau stage in E. coli.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 707
References
- ↑ What makes an Escherichia coli promoter sigma(S) dependent? Role of the -13/-14 nucleotide promoter positions and region 2.5 of sigma(S). Becker G, Hengge-Aronis R. Mol Microbiol, 2001 Mar;39(5):1153-65. PMID:11251833
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