Composite

Part:BBa_K3790223:Design

Designed by: Renbin Liu   Group: iGEM21_Fudan   (2021-10-16)


T7lac::RBS::Sso10b-(G2S)3-Bst::K395601


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 379
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The following primers were used for constructing this part. 

Bst-CE-f	5-CAGCGGCGCTGAGGGCGAAAAG
Bst-CE-r	5-CGGATCCTTATTTCGCATCGTAC
pET28b-CE-r	5-CCTCAGCGCCGCTGCTGTGATG
pET28b-CE-f	5-CGAAATAAGGATCCGAATTCGAG
29-f1		5-cagcagcggcGCTGAGGGCGAAAAGCCG   
29-r1		5-CGCCCTCAGCGCCGCTGCTGTGATGATGATG
29-r2		5-ATTCGGATCCTTATTTCGCATCGTACCAGG 
29-f2		5-GCGAAATAAGGATCCGAATTCGAGCTCCG  
29-r3		5-CCCTCAGCCATGGTATATCTCCTTC     
29-f3		5-GATATACCATGGCTGAGGGCGAAAAGCCG 
29-r4		5-AGCCGGATCCTTATTTCGCATCGTACCAGG
29-f4		5-GCGAAATAAGGATCCGGCTGCTAACAAAG 
pET28a-CE-r-noHis	5-CCTCAGCCATGGTATATCTCCTTC      
Bst-CE-f-noHis   	5-AGATATACCATGGCTGAGGGCGAAAAGC
Bst-CE-r         	5-CGGATCCTTATTTCGCATCGTACCAGGTC 
pET28a-CE-f      	5-GATGCGAAATAAGGATCCGAATTCGAG   
pET16b-CE-f     	5-GCGAAATAAGGATCCGGCTGCTAAC
Bst-CE-r2  	5-GCCTAGGTTAATTATTTCGCATCGTACC
pET52b-CE-f	5-CGAAATAATTAACCTAGGCTGCTGC


Source

References