Composite
Sso10b_Bst

Part:BBa_K3790160:Design

Designed by: Guanqiao Chi   Group: iGEM21_Fudan   (2021-10-16)


Sso10b-(G2S)3-Bst


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 289
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The following primers were used for constructing this part.

3-5-Sso10b-bst	5-GGTTGCACCGCTGCTAACGCCGCTGCTGTGATG                          
5-3-Sso10b-bst	5-GTTAGCAGCGGTGCAACC                                         
Sso10b-3-5-bst	5-CGCCGCTACCGCCGCTACCGCCGGCCTTTTTACGAATACCAATTTCAATG         
Sso10b-5-3-bst	5-TAGCGGCGGTAGCGGCGGTAGCGCTGAGGGCGAAAAG                      


Source

References