Coding
E.ssb
Part:BBa_K3790006:Design
Designed by: Guanqiao Chi Group: iGEM21_Fudan (2021-10-01)
E.ssb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The following primers were used for constructing this part.
E.ssb-1 5-GCAAGCCGTGGTGTTAATAAAGTTATTCTGGTGGGTAATCTGGGTCAAGATCCGGAAG E.ssb-2 5-GGGTCAAGATCCGGAAGTTCGTTATATGCCGAATGGTGGTGCAGTTGCAAATATTACCC E.ssb-3 5-GTGCAGTTGCAAATATTACCCTGGCAACCAGCGAAAGCTGGCGTGATAAAGCAACCGG E.ssb-4 5-CGTGATAAAGCAACCGGcGAAATGAAAGAACAGACCGAATGGCATCGTGTTGTTCTG E.ssb-5 5-GGCATCGTGTTGTTCTGTTTGGTAAACTGGCAGAAGTTGCAAGCGAATATCTGCG E.ssb-6 5-GCAAGCGAATATCTGCGTAAAGGTAGCCAGGTTTATATTGAAGGTCAGCTGCGTACCCG E.ssb-7 5-ACTTCGGTGGTATAACGATCCTGACCGCTCTGATCGGTCCATTTACGGGTACGCAGCTG E.ssb-8 5-CCACCTAACATCTGCATGGTGCCACCAACATTAACAACAACTTCGGTGGTATAACGATC E.ssb-9 5-ACCAATATTACCGCCTGCCGGTGCGCCACCACCTTGACGACCACCTAACATCTGCATGG E.ssb-10 5-GGCTGCTGAGGCTGACCCCAACCACCTTGCGGCTGACCACCACCAATATTACCGCCTGC E.ssb-11 5-TGCGGACGGCTCTGTGCACCACCGCTAAACTGGTTACCGCCTTGAGGCTGCTGAGGCTG E.ssb-12 5-CGGTTCATTGCTCGGTGCTGCTGGTGCGCTCTGCTGCGGACGGCTCTGTG E.ssb-13 5-AAACGGGATATCATCATCAAAATCCATAGGCGGTTCATTGCTCGGTGCTGC