Tag
Part:BBa_K3789007:Design
Designed by: MEI YING YI Group: iGEM21_UM_Macau (2021-09-26)
HA tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 58
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
HA-tag is used when detecting successfully expressed protein within yeast cells, due to its easy availability, we designed to use HA tag as an epitope to detect the protein of interest through western blot.
Source
Human influenza virus