Coding

Part:BBa_K3789005:Design

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-26)


Mating factor alpha prepro-leader (MF alpha)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 23
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 23
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 23
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 23
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since our proteins need to react with the substances in the surrounding media, we designed fusions of the MF alpha with the three genes SOD1, PNP1, and CHIT42. This sequence was commonly used in S. cerevisiae experiments, and it should reduce the rate of rejection of a foreign gene by the FY4 yeast strain.



Source

The sequence we used comes from Saccharomyces cerevisiae S288c


References