Regulatory

Part:BBa_K3789003:Design

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-26)


TPS1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 406


Design Notes

We chose TPS1 to induce the synthesis of endochitinase because endochitinase will destroy the cell wall, we need to control this process not to happen too early, the character of TPS1 makes the enzyme wouldn’t kill yeast too early.


Source

The sequence we used comes from Saccharomyces cerevisiae S288c


References