Coding
Part:BBa_K3788006:Design
Designed by: Rebecca Pagès Group: iGEM21_Aix-Marseille (2021-09-27)
ConA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 144
Illegal XhoI site found at 81 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 406
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.
Source
The ConA sequence was taken from uniprot sequence of ConA and liker was created by ourself