Coding
Part:BBa_K3787001:Design
Designed by: Leung Gabriel, Tam Ching Yeung, Wong Chun Hei Group: iGEM21_HK_GTC (2021-09-27)
WT PETase-MHETase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 348
Illegal PstI site found at 1889
Illegal PstI site found at 1918 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 304
Illegal NheI site found at 1038
Illegal PstI site found at 1889
Illegal PstI site found at 1918
Illegal NotI site found at 1586 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 348
Illegal PstI site found at 1889
Illegal PstI site found at 1918 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 348
Illegal PstI site found at 1889
Illegal PstI site found at 1918
Illegal NgoMIV site found at 1513
Illegal AgeI site found at 627 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
NA
Source
It is a chimeric protein. The part coding for both PETase and MHETase comes from the gene of the bacteria I. sakaiensis.