Part:BBa_K3784004
TAA1 (Encoding tryptophan aminotransferase)
TAA1 is thee gene from Arabidopsis thaliana, which involved in the conversion of tryptophan to 3-Indolepropionic acid. In the five-step metabolic pathway we reconstructed, tryptophan is first catalyzed by tryptophan aminotransferase encoded by TAA1 to produce indole pyruvate.
Characterization
We cloned TAA1 from Arabidopsis cDNA and inserted it into the His-6p-MBP-RSFD plasmid. TAA1 was initiated by a T7 promoter. After extracting total RNA, we obtained its cDNA by reverse transcription. We used cDNA as a template to amplify the fragment, then checked it by agarose gel electrophoresis. We chose rsmA (16S rRNA m(6)2A1518, m(6)2A1519 dimethyltransferase) as the internal reference gene and set a negative control. The TAA1 is well expressed and the expression levels of internal reference genes in the experimental group (rsmAE) and the control group (rsmAC) were consistent.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1227
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 69
Illegal XhoI site found at 1180 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 343
- 1000COMPATIBLE WITH RFC[1000]
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