Part:BBa_K3783017:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

OhioState Application

Figure 2. Endotoxin Units of Construct Lysates via LAL Assay

The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a slight increase in endotoxin activity in the pFraB-LF. However, when looking at the hybrid promoter the endotoxin activity was less than the control

User Reviews

UNIQ945089e40829cb7a-partinfo-00000001-QINU UNIQ945089e40829cb7a-partinfo-00000002-QINU