Part:BBa_K3783000:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

OhioState Application

The promoter was cloned into a luciferase reporter and then transformed into a strain of E.coli expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling the expression of proteins. The fraR repressor was expressed via the lacZ promoter, therefore the addition of IPTG results in increased repression.

Figure 1. pFraB Luciferase Reporter

User Reviews

UNIQ82a3568df4b5df7a-partinfo-00000001-QINU UNIQ82a3568df4b5df7a-partinfo-00000002-QINU