Part:BBa_K3782006:Design

Daucus carota ice-binding protein gene

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 279
  Illegal BglII site found at 347
  Illegal BglII site found at 824
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 833
  

Design Notes

The sequence was codon optimized for optimal expression in Escherichia coli.

Source

The source of DcAFP is Daucus carota. Its sequence was retrieved from GenBank. https://www.ncbi.nlm.nih.gov/nuccore/1139877302