Composite
Part:BBa_K3781201:Design
Designed by: Nicolas Bayer Group: iGEM21_TU_Kaiserslautern (2021-10-15)
L1_3xHA_RBD_mVenus, MocloMania Composite
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 619
Illegal PstI site found at 976 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 619
Illegal PstI site found at 976 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 557
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 619
Illegal PstI site found at 976 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 619
Illegal PstI site found at 976
Illegal NgoMIV site found at 705
Illegal NgoMIV site found at 1453 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 729
Design Notes
This composite part was assembled from three basic parts that are part of the MocloMania collection. Due to the highly standardizes set of overhangs that all our basic parts comply with, assembly could be achieved by a simple Moclo ligation. Assembling this part helped us to verify the basic parts' correct adaptation towards the employed MoClo cloning standard. Furthermore, immunostaining against the fused 3xHA tag allowed for evaluation of recombinant protein expression via western blot.
Source
For information on the origin of the genetic sequences present in this composite part, please consult the individual basic part pages.