Part:BBa_K3781008:Design
Myc + HRV 3C Motif, MocloMania B3
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This basic L0 part was de novo synthesized and thus easily codon optimized towards Leishmania tarentolae.
We generated this part by designing it in silico to carry the sequence of interest flanked by two invertedly oriented BbsI recognition sites as well as the desired B3 overhangs. After commercial synthesis, this allowed us to introduce the part into its respective L0 plasmid backbone with a simple MoClo ligation.
Source
The genetic sequence to this part was originally derived from a publically available Addgene plasmid #55183 named cMyc-tag.[1].
The c-myc gene as the underlying foundation to the tag's sequence is a human proto-oncogene and can thus be found within the human genome, NCBI Gene ID: 4609
The HRV 3C motif sequence is based on the recognition sequence of a human rhinovirus protease and can thus be found in human genes, e.g. coding for transcription factors.[2]
References
- ↑ Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T. J The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29. 10.1186/1754-1611-7-29 PubMed 24295448
- ↑ Amineva SP, Aminev AG, Palmenberg AC, Gern JE (October 2004). "Rhinovirus 3C protease precursors 3CD and 3CD' localize to the nuclei of infected cells". The Journal of General Virology. 85 (Pt 10): 2969–79. doi:10.1099/vir.0.80164-0. PMID 15448360