Part:BBa_K3781005:Design
sAP secretion tag, MocloMania B2
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 6
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence to this part was included in the commercially available pLEXSY_I-blecherry3 Leishmania expression vector.[1] As sAP1 is a Leishmania derived protein, the signal peptide did not have to be codon optimized. It was generated by running a PCR with specifically designed primers over the corresponding region in the pLEXSY_I-blecherry3 plasmid. These primers introduced two opposing BbsI recognition site at either end of the sequence as well as the specific B2 overhangs. Thus, the PCR product could be introduced into its respective L0 plasmid backbone with a simple MoClo ligation.
Source
The genetic sequence to this secretion peptide is included in the pLEXSY_I-blecherry3 expression vector provided by JenaBioscience[2]. It is originally derived from the gene for the secreted acid phosphatase 1 produced in Leishmania mexicana.[3]
References
- ↑ https://www.jenabioscience.com/lexsy-expression/lexsy-configurations/vectors/ege-243-plexsy_i-blecherry3
- ↑ https://www.jenabioscience.com/lexsy-expression/lexsy-configurations/inducible-genome-integrated/ege-1410blecherry-inducible-lexsy-expression-kit
- ↑ Ilg T, Stierhof YD, Etges R, Adrian M, Harbecke D, Overath P. Secreted acid phosphatase of Leishmania mexicana: a filamentous phosphoglycoprotein polymer. Proc Natl Acad Sci U S A. 1991;88(19):8774-8778. doi:10.1073/pnas.88.19.8774