Composite

Part:BBa_K3780017:Design

Designed by: Sumohit Sharma   Group: iGEM21_Stuttgart   (2021-09-27)


Dsup N-terminal His tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1589
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 203
    Illegal SpeI site found at 1590
    Illegal PstI site found at 1604
    Illegal NotI site found at 7
    Illegal NotI site found at 1597
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 142
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1590
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1590
    Illegal PstI site found at 1604
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The nucleotide sequence was optimised so that the amino acid sequence fits the E. Coli expression system. Prefix and Suffix sequence were add. The araBAD promoter was add after the prefix and in front of the DSUP, so that the expression of the DSUP is inducible by L-arabinose. For validation and purification a his tag was add at the N-term of Dsup.


Source

The sequence is available at NCBI.


References