Plasmid
Part:BBa_K3779004
Designed by: JiaCheng, Li Group: iGEM21_NWU-CHINA-B (2021-06-19)
pPICZαA
pPICZα is a terminal fusion vector. To express your gene as a recombinant fusion protein, you must clone your gene in frame with the N-terminal α-factor secretion signal and the C-terminal peptide containing the c-myc epitope and the polyhistidine tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1257
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BamHI site found at 1677
Illegal XhoI site found at 1184
Illegal XhoI site found at 1246 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1224
Illegal NgoMIV site found at 2435
Illegal NgoMIV site found at 2496
Illegal AgeI site found at 1396 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1660
Illegal BsaI.rc site found at 1859
Illegal BsaI.rc site found at 2857
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Categories
Parameters
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