Coding

Part:BBa_K377705:Design

Designed by: Marcus Lehr   Group: iGEM10_Michigan   (2010-10-26)

Flu (Ag43)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
    Illegal NgoMIV site found at 709
    Illegal NgoMIV site found at 1933
    Illegal NgoMIV site found at 2443
    Illegal NgoMIV site found at 2464
    Illegal AgeI site found at 1459
    Illegal AgeI site found at 2203
    Illegal AgeI site found at 2617
    Illegal AgeI site found at 2859
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has 6 PstI cut sites, and thus does not meet all BioBrick standards.

Source

[http://ecogene.org/geneInfo.php?eg_id=EG12686 EcoGene]

[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=full_report&list_uids=946540 NCBI]

References

[http://jb.asm.org/cgi/content/short/182/17/4789 Antigen 43 from Escherichia coli Induces Inter- and Intraspecies Cell Aggregation and Changes in Colony Morphology of Pseudomonas fluorescens]