Coding
Part:BBa_K377705:Design
Designed by: Marcus Lehr Group: iGEM10_Michigan (2010-10-26)
Flu (Ag43)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 883
Illegal PstI site found at 1441
Illegal PstI site found at 2041
Illegal PstI site found at 2665
Illegal PstI site found at 2936
Illegal PstI site found at 2989 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 883
Illegal PstI site found at 1441
Illegal PstI site found at 2041
Illegal PstI site found at 2665
Illegal PstI site found at 2936
Illegal PstI site found at 2989 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 883
Illegal PstI site found at 1441
Illegal PstI site found at 2041
Illegal PstI site found at 2665
Illegal PstI site found at 2936
Illegal PstI site found at 2989 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 883
Illegal PstI site found at 1441
Illegal PstI site found at 2041
Illegal PstI site found at 2665
Illegal PstI site found at 2936
Illegal PstI site found at 2989
Illegal NgoMIV site found at 709
Illegal NgoMIV site found at 1933
Illegal NgoMIV site found at 2443
Illegal NgoMIV site found at 2464
Illegal AgeI site found at 1459
Illegal AgeI site found at 2203
Illegal AgeI site found at 2617
Illegal AgeI site found at 2859 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has 6 PstI cut sites, and thus does not meet all BioBrick standards.
Source
[http://ecogene.org/geneInfo.php?eg_id=EG12686 EcoGene]
[http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=full_report&list_uids=946540 NCBI]
References
[http://jb.asm.org/cgi/content/short/182/17/4789 Antigen 43 from Escherichia coli Induces Inter- and Intraspecies Cell Aggregation and Changes in Colony Morphology of Pseudomonas fluorescens]