Coding
cas8

Part:BBa_K3776013

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-01)


cas8

This Cas8 protein from Vibrio cholerae is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering (see BBa_K3776022). In this system, Cas8 is part of the TniQ-Cascade protein complex which is necessary for RNA-guided DNA integration.(Klompe et al., 2019)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 434
    Illegal BglII site found at 964
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1720


[edit]
Categories
Parameters
None