Part:BBa_K3776005:Design

AK T369I

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1173
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 145
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part was designed by Qi et al.(2011) and has been codon optomized for the use in Synechococcus elongatus UTEX 2973.

Source

It was mutated from AK from Xenorhabdus bovienii by Qi et al.(2011).

References

Qi, Q., Huang, J., Crowley, J., Ruschke, L., Goldman, B. S., Wen, L., & Rapp, W. D. (2011). Metabolically engineered soybean seed with enhanced threonine levels: biochemical characterization and seed-specific expression of lysine-insensitive variants of aspartate kinases from the enteric bacterium Xenorhabdus bovienii. Plant Biotechnology Journal, 9(2), 193–204. https://doi.org/10.1111/j.1467-7652.2010.00545.x

Korosh, T. C., Markley, A. L., Clark, R. L., McGinley, L. L., McMahon, K. D., & Pfleger, B. F. (2017). Engineering photosynthetic production of L-lysine. Metabolic Engineering, 44, 273–283. https://doi.org/10.1016/j.ymben.2017.10.010