Part:BBa_K3773514:Experience

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Please enter how you used this part and how it worked out.

iGEM21_William_and_Mary

Our initial confirmation of this part's function was as follows. In order to test the functionality of WM21_014, our team performed a series of tests to determine fluorescence. As this system is supposed to fluoresce in response to changes in osmolarity, our team planned to grow this circuit in LB media with a 20% sucrose concentration overnight. After overnight growth, our team planned to measure the fluorescence of the culture with a plate reader. However, after our transformations, our team encountered an issue. Our colonies transformed with WM21_014 were fluorescent on the plate without having been induced. Obviously, our circuit was not working properly and our team decided to design a new circuit.

User Reviews

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