Part:BBa_K3773010:Design

lon promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This sequence has previously been successfully fused to GFP and fluorescence was quantified to measure heat shock response in E. coli¹. Additionally, the sequence (albeit possibly with a different beginning site for the promoter) has been successfully fused to lacZ in order to quantify expression levels².

When placed before sfGFP in our hands, this promoter successfully displayed fluorescence. For more information about functionally confirming the composite part containing this promoter, please see the "Experience" section of the page for BBa_K3773523.

Source

We derived the sequence from two sources, one of which provided the sequence of bases², and the other of which allowed us to determine a beginning site for the promoter sequence from the primer sequence used¹.

References

¹Aertsen, A., Vanoirbeek, K., De Spiegeleer, P., Sermon, J., Hauben, K., Farewell, A., ... & Michiels, C. W. (2004). Heat shock protein-mediated resistance to high hydrostatic pressure in Escherichia coli. Applied and Environmental Microbiology, 70(5), 2660-2666.

²Chin, D. T., Goff, S. A., Webster, T., Smith, T., & Goldberg, A. L. (1988). Sequence of the lon gene in Escherichia coli. A heat-shock gene which encodes the ATP-dependent protease La. Journal of Biological Chemistry, 263(24), 11718-11728.