Part:BBa_K3773007:Design

clpB RBS native to E. coli

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This RBS was successfully used with the native clpB promoter in a circuit used to detect heat shock response through GFP fluorescence¹ and through lacZ².

Source

Kitagawa, M. A. S. A. N. A. R. I., Wada, C., Yoshioka, S., & Yura, T. (1991). Expression of ClpB, an analog of the ATP-dependent protease regulatory subunit in Escherichia coli, is controlled by a heat shock sigma factor (sigma 32). Journal of Bacteriology, 173(14), 4247-4253.

Cha, H. J., Srivastava, R., Vakharia, V. N., Rao, G., & Bentley, W. E. (1999). Green fluorescent protein as a noninvasive stress probe in resting Escherichia coli cells. Applied and Environmental Microbiology, 65(2), 409-414.

References

¹Kitagawa, M. A. S. A. N. A. R. I., Wada, C., Yoshioka, S., & Yura, T. (1991). Expression of ClpB, an analog of the ATP-dependent protease regulatory subunit in Escherichia coli, is controlled by a heat shock sigma factor (sigma 32). Journal of Bacteriology, 173(14), 4247-4253.

²Cha, H. J., Srivastava, R., Vakharia, V. N., Rao, G., & Bentley, W. E. (1999). Green fluorescent protein as a noninvasive stress probe in resting Escherichia coli cells. Applied and Environmental Microbiology, 65(2), 409-414.