Part:BBa_K3773006:Design
clpB promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 235
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This promoter was successfully used as in a similar circuit used to detect heat shock response through GFP fluorescence.
Source
Kitagawa, M. A. S. A. N. A. R. I., Wada, C., Yoshioka, S., & Yura, T. (1991). Expression of ClpB, an analog of the ATP-dependent protease regulatory subunit in Escherichia coli, is controlled by a heat shock sigma factor (sigma 32). Journal of Bacteriology, 173(14), 4247-4253.
Squires, C. L., Pedersen, S., Ross, B. M., & Squires, C. (1991). ClpB is the Escherichia coli heat shock protein F84. 1. Journal of bacteriology, 173(14), 4254-4262.
Cha, H. J., Srivastava, R., Vakharia, V. N., Rao, G., & Bentley, W. E. (1999). Green fluorescent protein as a noninvasive stress probe in resting Escherichia coli cells. Applied and Environmental Microbiology, 65(2), 409-414.