Composite
Part:BBa_K3772010:Design
Designed by: Xiaoyu Wu Group: iGEM21_SCUT-China (2021-10-01)
PDC1p-VS-SAG1t expression cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1598
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 302
Design Notes
This sequence is codon optimized by Sangon Biotech. (https://www.sangon.com/services_gene_mmzyhhjysjfw.html) The parts are connected together by enzyme digestion
Source
PDC1p and SAG1t are cloned from the promoter of the coding sequence for aldehyde dehydrogenase from the genome of saccharomyces cereviciae BJ5464-NpgA; VS is synthesized by Sangon Biotech according to GenBank:JX040471.1;