Composite

Part:BBa_K3766108:Design

Designed by: Kamyar Keshavarz-Farajkhah   Group: iGEM21_Evry_Paris-Saclay   (2021-09-25)


pTet evoAPOBEC1-BE4max T7RNAP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 828
    Illegal EcoRI site found at 1068
    Illegal XbaI site found at 706
    Illegal PstI site found at 1392
    Illegal PstI site found at 1452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 828
    Illegal EcoRI site found at 1068
    Illegal PstI site found at 1392
    Illegal PstI site found at 1452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 828
    Illegal EcoRI site found at 1068
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 828
    Illegal EcoRI site found at 1068
    Illegal XbaI site found at 706
    Illegal PstI site found at 1392
    Illegal PstI site found at 1452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 828
    Illegal EcoRI site found at 1068
    Illegal XbaI site found at 706
    Illegal PstI site found at 1392
    Illegal PstI site found at 1452
    Illegal AgeI site found at 747
    Illegal AgeI site found at 777
    Illegal AgeI site found at 1440
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

to do


Source

DNA synthesis, PCR and assembly

References