RBS

Part:BBa_K3766032:Design

Designed by: Doriane Blaise   Group: iGEM21_Evry_Paris-Saclay   (2021-09-25)


RBS from pET26b with XbaI and SalI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

derived from pSEVA221AID-T7RNAP, GenBank Acc n°MN450172

Source

PCR amplification from pSEVA221AID-T7RNAP and assembly

References