Coding

Part:BBa_K3766012:Design

Designed by: Doriane Blaise   Group: iGEM21_Evry_Paris-Saclay   (2021-09-25)


AmpR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To disrupt the BsaI recognition site GGTCTC by site-directed mutagenesis we choose to change its sequence to GGTCGC. Care was taken that the mutation should not affect the amino acid composition of the protein. The chosen mutation is a synonymous one TCT(Ser) to TCG(Ser). 

                     >BsaI
                      |
pSB1A2:       1103 - GGG TCT CGC - 1095
                      G   S   R

BBa K3766012:  715 - GGG TCG CGC - 723
                      G   S   R


Source

PCR amplification from a BsaI-free version of pSB1A2

References