Coding

Part:BBa_K3766008:Design

Designed by: Doriane Blaise   Group: iGEM21_Evry_Paris-Saclay   (2021-09-25)


evoAPOBEC1-BE4max T7RNAP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 94
    Illegal EcoRI site found at 334
    Illegal PstI site found at 658
    Illegal PstI site found at 718
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 94
    Illegal EcoRI site found at 334
    Illegal PstI site found at 658
    Illegal PstI site found at 718
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 94
    Illegal EcoRI site found at 334
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 94
    Illegal EcoRI site found at 334
    Illegal PstI site found at 658
    Illegal PstI site found at 718
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 94
    Illegal EcoRI site found at 334
    Illegal PstI site found at 658
    Illegal PstI site found at 718
    Illegal AgeI site found at 13
    Illegal AgeI site found at 43
    Illegal AgeI site found at 706
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TO DO


Source

DNA synthesis and assembly

References