Translational_Unit

Part:BBa_K3765019

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)


T7 RBS + OpdA+SpyTag002 without OpdA signal peptide

Translational Unit comprising of the CDS of an OpdA-SpyTag002 (without OpdA signal peptide) fusion protein under a strong RBS from bacteriophage T7 gene 10.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1171
    Illegal AgeI site found at 1234
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/degradation
Parameters
None