Coding

Part:BBa_K3765010:Design

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)


sfGFP (Superfolder GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for E. coli.

Source

Engineered from the native GFP of Aequorea victoria.

References

  1. Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88. doi: 10.1038/nbt1172.