Coding

Part:BBa_K3765008

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)


OpdA

Phosphotriesterase enzyme from Agrobacterium tumefaciens, which degrades organophosphates which are triesters of phosphoric acid. Unlike the previous OpdA part on the Registry BBa_K215090, this part also encodes the 17 amino acid long tail at the C-terminus of the enzyme which may be useful for construction of C-terminal fusion proteins using this part.

Usage and Biology

This part was of major importance in our project. The OpdA enzyme can degrade a wide range of organophosphates and since our project is aimed at building a filter to fight organophosphate pollution, we constructed an OpdA-SpyTag002 fusion protein to functionalize a microcrystalline cellulose sheet-based filter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/degradation
Parameters
None