Measurement

Part:BBa_K376017:Experience

Designed by: Anisha Katyal, Andrew Kirk, Erik McCann, Lauren Rossi, Becky Hennessey   Group: iGEM10_Penn_State   (2010-10-27)

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Applications of BBa_K376017

This Lux promoter tester was used in an experiment to measure the effectiveness of AHL as an inducer in Lux promoters. The LuxR was under constitutive expression so that the limiting factor would be AHL. The coding sequences for LuxR and RFP were preceded by the standard RBS B0034.

The AHL used was OC6-homoserine lactone. It was added to samples in a 96-well plate in concentrations of 0, .1, 1, 10, 100 and 1000nM.

The results [http://2010.igem.org/Team:Penn_State/Project#Lux_Promoter_Characterization on our wiki] show no trend in protein expression based on concentration of OC6-homoserine lactone. More research is warranted to provide information about what are the best conditions for chemically inducing the Lux promoters.

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User Reviews

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