Part:BBa_K376004:Design
Anaerobic Fluorescent Protein with RBS
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This protein was codon optimized for E. coli by DNA 2.0 and the the RBS was optimized using the Salis Lab RBS calculator http://salis.psu.edu/RBS_Calculator.shtml
Source
Protein sequence was taken from:
Tielker D, Eichhof I, Jaeger KE, Ernst JF. Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae. Eukaryot Cell. 2009 (6):913-915
References
Tielker D, Eichhof I, Jaeger KE, Ernst JF. 2009. Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae. Eukaryot Cell. 2009 (6):913-915
Salis HM, Mirsky EA, Voigt CA. 2009. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol. 2009 Oct;27(10):946-50