Coding

Part:BBa_K3755045:Design

Designed by: Qi Xin   Group: iGEM21_ShanghaiTech_China   (2021-10-13)


Hok/Mok protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is the coding sequence of a toxic protein, put it downstream of a controllable promoter to make a kill switch for bactria.


Source

Isolated from R1 plasmid of E.coli.

References

[1]Kawano M. Divergently overlapping cis-encoded antisense RNA regulating toxin-antitoxin systems from E. coli: hok/sok, ldr/rdl, symE/symR. RNA Biol. 2012 Dec;9(12):1520-7. doi: 10.4161/rna.22757. Epub 2012 Nov 6. PMID: 23131729.