Coding
Part:BBa_K3755045:Design
Designed by: Qi Xin Group: iGEM21_ShanghaiTech_China (2021-10-13)
Hok/Mok protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is the coding sequence of a toxic protein, put it downstream of a controllable promoter to make a kill switch for bactria.
Source
Isolated from R1 plasmid of E.coli.
References
[1]Kawano M. Divergently overlapping cis-encoded antisense RNA regulating toxin-antitoxin systems from E. coli: hok/sok, ldr/rdl, symE/symR. RNA Biol. 2012 Dec;9(12):1520-7. doi: 10.4161/rna.22757. Epub 2012 Nov 6. PMID: 23131729.