Tag

Part:BBa_K3755009:Design

Designed by: Qi Xin   Group: iGEM21_ShanghaiTech_China   (2021-09-30)


ATKA expression tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To use AKTK expression tag to improve yield of protein, you must put it immediately following the start codon and the target protein sequence is placed downstream of the tag.

Source

From literature of Eugene Kim's team from Washington University in St. Louis.

References

[1]Tuller T, Carmi A, Vestsigian K, Navon S, Dorfan Y, Zaborske J, Pan T, Dahan O, Furman I, Pilpel Y. An evolutionarily conserved mechanism for controlling the efficiency of protein translation. Cell. 2010 Apr 16;141(2):344-54. doi: 10.1016/j.cell.2010.03.031. PMID: 20403328.

[2]Kim E, Dai B, Qiao JB, Li W, Fortner JD, Zhang F. Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces. 2018 Dec 12;10(49):43003-43012. doi: 10.1021/acsami.8b14890. Epub 2018 Nov 27. PMID: 30480422.