Coding

Part:BBa_K3755006:Design

Designed by: Kaijun Wang   Group: iGEM21_ShanghaiTech_China   (2021-09-29)


mouse Piezo1.1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3555
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2975
    Illegal BglII site found at 7388
    Illegal BamHI site found at 4125
    Illegal XhoI site found at 7000
    Illegal XhoI site found at 7207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 790
    Illegal NgoMIV site found at 1771
    Illegal NgoMIV site found at 1798
    Illegal NgoMIV site found at 2843
    Illegal NgoMIV site found at 3068
    Illegal NgoMIV site found at 5750
    Illegal NgoMIV site found at 7312
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 877
    Illegal BsaI.rc site found at 4645
    Illegal BsaI.rc site found at 7471
    Illegal BsaI.rc site found at 7537
    Illegal SapI site found at 3741
    Illegal SapI site found at 6549
    Illegal SapI.rc site found at 3984
    Illegal SapI.rc site found at 4690
    Illegal SapI.rc site found at 5413


Design Notes

All of the introns have been removed in this sequence. Termination condon is not included in sequence.

Source

The pcDNA3.1 plasmid containing mPiezo1.1 was obtained from Bailong Xiao'lab, Tsinghua university. mPiezo1.1 is a splicing variant of mPiezo1 which is identified in C2C12 cell line with RNA-seq.

References

[1]Geng J, Liu W, Zhou H, Zhang T, Wang L, Zhang M, Li Y, Shen B, Li X, Xiao B. A Plug-and-Latch Mechanism for Gating the Mechanosensitive Piezo Channel. Neuron. 2020 May 6;106(3):438-451.e6. doi: 10.1016/j.neuron.2020.02.010. Epub 2020 Mar 5. PMID: 32142647.
[2]Lacroix JJ, Botello-Smith WM, Luo Y. Probing the gating mechanism of the mechanosensitive channel Piezo1 with the small molecule Yoda1. Nat Commun. 2018 May 23;9(1):2029. doi: 10.1038/s41467-018-04405-3. PMID: 29795280; PMCID: PMC5966384.
[3]Botello-Smith WM, Jiang W, Zhang H, Ozkan AD, Lin YC, Pham CN, Lacroix JJ, Luo Y. A mechanism for the activation of the mechanosensitive Piezo1 channel by the small molecule Yoda1. Nat Commun. 2019 Oct 3;10(1):4503. doi: 10.1038/s41467-019-12501-1. PMID: 31582801; PMCID: PMC6776524.