Regulatory

Part:BBa_K3753012:Design

Designed by: Yiqing Zhang   Group: iGEM21_NJTech_China   (2021-10-16)


UASCIT-pTEF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 845
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 845
    Illegal NgoMIV site found at 1367
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

UASCIT-pTEF is synthesized by fusing three UASCIT tandem to the tef2 promoter (BBa_K3753003). UASCIT was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 275bp sequence upstream of the cit2 promoter start codon.

Source

Saccharomyces cerevisiae

References

John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895.

Mark Rosenkrantz et al. Distinct upstream activation regions for glucose-repressed and derepressed expression of the yeast citrate synthase gene CIT1[J]. Current Genetics, 1994, 25(3) : 185-195.