RNA
Part:BBa_K3752000:Design
Designed by: Tudor Onose Group: iGEM21_Warwick (2021-10-20)
OXA-48 Parent cgRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The RNA had to be engineered to have a series of domains which would cause the dCas9 binding handle to be misfolded in its native state. When the OXA-48 is present however, the two mRNAs anneal in such a way that the dCas9 binding handle is folded correctly.
Source
Designed