Coding

Part:BBa_K3748006:Design

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


Secreted Alpha amylase from Bacillus subtilis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 607
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 607
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 607
    Illegal BglII site found at 1079
    Illegal BglII site found at 1249
    Illegal BamHI site found at 1099
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 607
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 607
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Secreted protein, codon optimized and Golden Gate Assembly suitable.

Source

Genomic data from European Nucleotide Archive.

References

https://www.uniprot.org/uniprot/P00691