Other

Part:BBa_K3747605

Designed by: Sanne van Doorn   Group: iGEM21_Wageningen_UR   (2021-10-20)


lox2m/71

lox site lox2m/71, synthetic

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Lox2m/71 is an engineered version of the wild-type loxP. Cre recombinase recognizes this specific lox site and consequently mediates site-specific recombination between two compatible lox sites [1]. The exact sequence was retrieved from [2].

Functional Parameters

References

[1]Design of DNA-binding peptides based on the leucine zipper motif. O'Neil KT, Hoess RH, DeGrado WF Science. 1990 Aug 17; 249(4970):774-8.

[2] Garcia-Morales, L., Ruiz, E., Gourgues, G., Rideau, F., Piñero-Lambea, C., Lluch-Senar, M., ... & Lartigue, C. (2020). A RAGE Based Strategy for the Genome Engineering of the Human Respiratory Pathogen Mycoplasma pneumoniae. ACS synthetic biology,/i>, 9(10), 2737-2748.

[edit]
Categories
Parameters
None