Coding

Part:BBa_K3747603:Design

Designed by: Sanne van Doorn   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nos_operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1030
    Illegal PstI site found at 1330
    Illegal PstI site found at 1600
    Illegal PstI site found at 2800
    Illegal PstI site found at 2884
    Illegal PstI site found at 4107
    Illegal PstI site found at 5790
    Illegal PstI site found at 6249
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1030
    Illegal NheI site found at 1540
    Illegal PstI site found at 1330
    Illegal PstI site found at 1600
    Illegal PstI site found at 2800
    Illegal PstI site found at 2884
    Illegal PstI site found at 4107
    Illegal PstI site found at 5790
    Illegal PstI site found at 6249
    Illegal NotI site found at 6674
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1030
    Illegal BglII site found at 1970
    Illegal BglII site found at 3779
    Illegal XhoI site found at 334
    Illegal XhoI site found at 1099
    Illegal XhoI site found at 4823
    Illegal XhoI site found at 5970
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1030
    Illegal PstI site found at 1330
    Illegal PstI site found at 1600
    Illegal PstI site found at 2800
    Illegal PstI site found at 2884
    Illegal PstI site found at 4107
    Illegal PstI site found at 5790
    Illegal PstI site found at 6249
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1030
    Illegal PstI site found at 1330
    Illegal PstI site found at 1600
    Illegal PstI site found at 2800
    Illegal PstI site found at 2884
    Illegal PstI site found at 4107
    Illegal PstI site found at 5790
    Illegal PstI site found at 6249
    Illegal NgoMIV site found at 258
    Illegal NgoMIV site found at 591
    Illegal NgoMIV site found at 3121
    Illegal NgoMIV site found at 3334
    Illegal NgoMIV site found at 3912
    Illegal NgoMIV site found at 4459
    Illegal NgoMIV site found at 5254
    Illegal AgeI site found at 1909
    Illegal AgeI site found at 2344
    Illegal AgeI site found at 3156
    Illegal AgeI site found at 4990
    Illegal AgeI site found at 5134
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The structure of this Nos operon is nosRZDFYL, tatA. More information on the subunits can be found here:

nosR: BBa_K3747117,nosZ:BBa_K3747118,nosD:BBa_K3747119,nosF:BBa_K3747120,nosY:BBa_K3747121,nosL:BBa_K3747122,,tatA: BBa_K3747123,

Source

Pseudomonas stutzeri JM300

References