Coding
Part:BBa_K3747602:Design
Designed by: Sanne van Doorn Group: iGEM21_Wageningen_UR (2021-10-20)
Nor_operon
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2529
Illegal PstI site found at 3654
Illegal PstI site found at 4136
Illegal PstI site found at 4601
Illegal PstI site found at 4931
Illegal PstI site found at 4952 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2529
Illegal PstI site found at 3654
Illegal PstI site found at 4136
Illegal PstI site found at 4601
Illegal PstI site found at 4931
Illegal PstI site found at 4952 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3571
Illegal BamHI site found at 4405 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2529
Illegal PstI site found at 3654
Illegal PstI site found at 4136
Illegal PstI site found at 4601
Illegal PstI site found at 4931
Illegal PstI site found at 4952 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2529
Illegal PstI site found at 3654
Illegal PstI site found at 4136
Illegal PstI site found at 4601
Illegal PstI site found at 4931
Illegal PstI site found at 4952
Illegal NgoMIV site found at 700
Illegal NgoMIV site found at 1470
Illegal NgoMIV site found at 3741
Illegal NgoMIV site found at 4148
Illegal NgoMIV site found at 4283
Illegal NgoMIV site found at 4858
Illegal NgoMIV site found at 5028
Illegal AgeI site found at 1199
Illegal AgeI site found at 3180 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The structure of this Nor operon is nirQOP,norCBC. More information for the subunits can be found here:nirQ:BBa_K3747133,nirO:BBa_K3747134,nirP:BBa_K3747135, norC:BBa_K3747130,norB:BBa_K3747131,, norD:BBa_K3747132,
Source
Pseudomonas stutzeri JM300