Coding

Part:BBa_K3747602:Design

Designed by: Sanne van Doorn   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nor_operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2529
    Illegal PstI site found at 3654
    Illegal PstI site found at 4136
    Illegal PstI site found at 4601
    Illegal PstI site found at 4931
    Illegal PstI site found at 4952
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2529
    Illegal PstI site found at 3654
    Illegal PstI site found at 4136
    Illegal PstI site found at 4601
    Illegal PstI site found at 4931
    Illegal PstI site found at 4952
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3571
    Illegal BamHI site found at 4405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2529
    Illegal PstI site found at 3654
    Illegal PstI site found at 4136
    Illegal PstI site found at 4601
    Illegal PstI site found at 4931
    Illegal PstI site found at 4952
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2529
    Illegal PstI site found at 3654
    Illegal PstI site found at 4136
    Illegal PstI site found at 4601
    Illegal PstI site found at 4931
    Illegal PstI site found at 4952
    Illegal NgoMIV site found at 700
    Illegal NgoMIV site found at 1470
    Illegal NgoMIV site found at 3741
    Illegal NgoMIV site found at 4148
    Illegal NgoMIV site found at 4283
    Illegal NgoMIV site found at 4858
    Illegal NgoMIV site found at 5028
    Illegal AgeI site found at 1199
    Illegal AgeI site found at 3180
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The structure of this Nor operon is nirQOP,norCBC. More information for the subunits can be found here:nirQ:BBa_K3747133,nirO:BBa_K3747134,nirP:BBa_K3747135, norC:BBa_K3747130,norB:BBa_K3747131,, norD:BBa_K3747132,

Source

Pseudomonas stutzeri JM300

References