Coding

Part:BBa_K3747118:Design

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nitrous oxide reductase Z (nosZ) from Pseudomonas stutzeri JM300


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 544
    Illegal PstI site found at 628
    Illegal PstI site found at 1851
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 544
    Illegal PstI site found at 628
    Illegal PstI site found at 1851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1523
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 544
    Illegal PstI site found at 628
    Illegal PstI site found at 1851
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 544
    Illegal PstI site found at 628
    Illegal PstI site found at 1851
    Illegal NgoMIV site found at 865
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 1656
    Illegal AgeI site found at 88
    Illegal AgeI site found at 900
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Pseudomonas stutzeri JM300

References