Translational_Unit

Part:BBa_K3745034

Designed by: Jianfei Song   Group: iGEM21_BNDS_China   (2021-09-30)


Protein scaffold for rhlA,B,C complex

This part codes for the GBD, SH3, PDZ domain that consists of the protein scaffold. These three domains bind with GBD-tag, SH3-tag and PDZ-tag in front of rhlA (see BBa_K3745031), rhlB (see BBa_K3745032) and rhlC (see BBa_K3745033) to connect rhlA,B,C to the scaffold.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1790
    Illegal XhoI site found at 1799
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 596
    Illegal NgoMIV site found at 911
    Illegal NgoMIV site found at 1226
    Illegal NgoMIV site found at 1541
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 826
    Illegal BsaI site found at 1141
    Illegal BsaI site found at 1456
    Illegal BsaI site found at 1771
    Illegal SapI.rc site found at 455


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Categories
Parameters
None