Composite

Part:BBa_K374009:Design

Designed by: Lisa Blanc Iversen, Maya Friis Kjaergaard, Annemi Jollmann, Anja Sander and Grzegorz Słodkowicz   Group: iGEM10_DTU-Denmark   (2010-10-20)

Gifsy2 promoters and Gifsy2 repressor gtgR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains both promoters and repressor. We chose not to separate the repressor gene from the promoter because we wanted to keep the natural RBS.



Source

Genomic sequence from the Salmonella typhimurium ATCC 14028

References

Lemire, S. et al. A Singular Case of Prophage Complementation in Mutational Activation of recET orthologs in Salmonella enterica Serovar Typhimurium. Journal of Bacteriology 20, 6857-6866, (2008)