Regulatory

Part:BBa_K374005:Design

Designed by: Patrick Fortuna, Thomas Trolle, Anastasiya S. Haugaard, Martin Malthe Borch, Juliet Frederiksen   Group: iGEM10_DTU-Denmark   (2010-10-17)

Lambda nutR site - N utilization site + Tr1 rho dependent terminator.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Requires a couple hundred base pairs upstream of the terminator.


Source

nutR site from the lambda phage from E.coli.

References

[1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768.